JOURNAL OF CULTURE COLLECTIONS
Volume 6, 2008-2009, pp. 61-68
IDENTIFICATION OF STRAINS ISOLATED IN THAILAND AND ASSIGNED TO THE GENERA KOZAKIA AND SWAMINATHANIA
Jintana Kommanee1, Somboon Tanasupawat1,*, Ancharida Akaracharanya2, Taweesak Malimas3, Pattaraporn Yukphan3, Yuki Muramatsu4, Yasuyoshi Nakagawa4 and Yuzo Yamada3,†
1Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand;
2Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; 3BIOTEC Culture Collection, National Center for Genetic Engineering and Biotechnology, Pathumthani 12120, Thailand;
4Biological Resource Center, Department of Biotechnology, National Institute of Technology and Evaluation, Kisarazu 292-0818, Japan;
†JICA Senior Overseas Volunteer, Japan International Cooperation Agency, Shibuya-ku, Tokyo 151-8558, Japan; Professor Emeritus, Shizuoka University, Suruga-ku, Shizuoka 422-8529, Japan
*Corresponding author, e-mail: Somboon.T@chula.ac.th
Summary
Four isolates, isolated from fruit of sapodilla collected at Chantaburi and designated as CT8-1 and CT8-2, and isolated from seeds of ixora („khem” in Thai, Ixora species) collected at Rayong and designated as SI15-1 and SI15-2, were examined taxonomically. The four isolates were selected from a total of 181 isolated acetic acid bacteria. Isolates CT8-1 and CT8-2 were non motile and produced a levan-like mucous polysaccharide from sucrose or D-fructose, but did not produce a water-soluble brown pigment from D-glucose on CaCO3-containing agar slants. The isolates produced acetic acid from ethanol and oxidized acetate and lactate to carbon dioxide and water, but the intensity of the acetate and lactate oxidation was weak. Their growth was not inhibited by 0.35 % acetic acid (v/v) at pH 3.5. The isolates did not grow on 30 % D-glucose (w/v), and utilization of methanol was not found. Isolates SI15-1 and SI15-2 had peritrichous flagella and grew in the presence of either 0.35 % acetic acid (v/v) at pH 3.5, 3 % NaCl (w/v), or 1 % KNO3 (w/v). Acetate and lactate were oxidized to carbon dioxide and water, but the intensity was weak. The isolates grew on mannitol agar and glutamate agar as well as on 30 % D-glucose (w/v), but did not utilize methanol. The 16S rRNA gene sequence analysis and DNA-DNA hybridization indicated that isolates CT8-1 and CT8-2 and isolates SI15-1 and SI15-2 were unequivocally identified respectively as Kozakia baliensis and Swaminathania salitolerans.
Key words: acetic acid bacteria, Alphaproteobacteria, Kozakia baliensis, Swaminathania salitolerans, 16S rRNA gene sequences