JOURNAL OF
CULTURE COLLECTIONS
Volume 6, 2008-2009,
pp. 106-111
Serological characterization of paprika mild mottle tobamovirus strain P101
Angela Yordanova1* and Elisaveta Stoimenova2
1National Bank for Industrial Microorganisms and Cell Cultures, P.O.Box 239, 1113 Sofia, Bulgaria;
2Institute of Genetics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria
*Corresponding author, e-mail: ayordanova@dir.bg
Summary
The serological properties of the Bulgarian strain P101 of paprika mild mottle virus (PAMMV) were investigated. Counter immunoelectrophoresis and double diffusion test were applied. The serological relationship with tobacco mosaic virus (TMV), tomato mosaic virus (ToMV), tobacco mild green mosaic virus (TMGMV), pepper mild mottle virus (PMMoV) and the Dutch strain P11 of PaMMV was studied using antisera against P101 and ToMV. The Bulgarian strain P101 was serologically very closely related to the PaMMV strain P11 originated from the Netherlands. The performed immunodiffusion tests with homologous and heterologous antisera allow P101 to be arranged in different series of serological relationship with typical tobamovirus strains. PaMMV is more related to TMGMV and PMMoV than to TMV and ToMV.
Key words: paprika mild mottle virus, tobamoviruses, immunodiffusion, mmunoelectrophoresis, serological relationship