JOURNAL
OF CULTURE COLLECTIONS
Volume 5, 2006-2007,
pp. 58-63
ISOLATION OF BACTERIOCINOGENIC LACTIC ACID BACTERIA
FROM RAT INTESTINE
Mahantesh Patil*, Ajay Pal, Vijai Pal and Rajini Kumari Yaddula
Food Biotechnology Discipline, Defence Food Research Laboratory,
Siddartha
Nagar, Mysore-570011,
*Corresponding author, e-mail: dfrlmysore@sancharnet.in
Summary
Lactic
acid bacteria were isolated from rat intestinal contents and screened for
bacteriocin production. Out of twenty-three isolates, eight were found to
produce bacteriocins. The morphological and biochemical properties of these
isolates indicated that they belong to the genus Enterococcus. The bacteriocins were extracted and partially
purified by Gel Permeation Chromatography (GPC)
using G-25, and the antimicrobial spectrum of these
bacteriocins was studied against indicator organisms, including Lactococcus diacetylactis, Staphylococcus
aureus, Escherichia coli and Pseudomonas sp.
Key words: lactic acid bacteria, bacteriocins, Enterococcus, intestine.