JOURNAL OF CULTURE COLLECTIONS

Volume 5, 2006-2007, pp. 35-37

EXTRA- AND INTRACELLULAR ESTERASES INVOLVED IN DIMETHYLTEREPHTHALATE CATABOLISM BY PSEUDOMONAS SP.

Lilia Tserovska^1*, Raycho Dimkov², Tanya Rasheva³ and Tanya Yordanova¹

¹National Bank for Industrial Microorganisms and Cell Cultures;1113 Sofia, P.O.Box 239, Bulgaria;
²Sofia University, Faculty of Biology, Department of Hydrobiology and Ichthyology,
8 Dragan Tzankov blvd., 1164 Sofia, Bulgaria;
³Sofia University, Faculty of Biology, Department of Microbiology, 8 Dragan Tzankov blvd., 1164 Sofia, Bulgaria

*Corresponding author, e-mail: l_tserovska@abv.bg

Summary

The strain Pseudomonas sp. 054, isolated previously from polluted soil, possesses a high biodegrading activity towards the aromatic ester dimethylterephthalate. The ester hydrolyzing activity of the strain was investigated. Two types of esterases, extra- and intracellular, were detected after growth in liquid medium. The enzyme activities were proved in the culture supernatant and in the crude cell extract by the use of a native electrophoresis. The extracellular protein with an esterase activity had a molecular weight of 67 kDa, while the intracellular fraction produced four separate bands with ester hydrolyzing activity.

Key words: Pseudomonas, dimethylterephthalate, esterases.

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