JOURNAL OF CULTURE COLLECTIONS
Volume 5, 2006-2007, pp. 3-9
TAXONOMIC CHARACTERIZATION
OF THE THERMOPHILIC ACTINOMYCETE STRAIN 21E –
PRODUCER OF THERMOSTABLE COLLAGENASE
Detelina Petrova* and Stoyan Vlahov
8 Dragan Tzankov str., 1164
*Corresponding author, e-mail: detelina@biofac.uni-sofia.bg
Summary
The thermophilic
actinomycete strain 21E isolated from saline Bulgarian soils produced a highly
thermostable collagenase. Macro- and micromorphological characteristics of the
strain were tested on fourteen media. Some physiological and biochemical
properties, enzyme activities, as well as the antibiotic activity and sensitivity
were examined. It was concluded that the strain 21E was a typical member of the
genus Thermoactinomyces. The comparative references to Thermoactinomyces
species with similar taxonomic characteristics identified the strain as
Thermoactinomyces sacchari. T. sacchari strain 21E was resistant to the
antibiotic novobiocine, and displayed protease, amylase, collagenase and lipase
activities.
Key words: taxonomy,
thermophilic actinomycetes, Thermoactinomyces sacchari, soil, collagenase