JOURNAL OF CULTURE COLLECTIONS
Volume 2, 1997-1998

 

AN INCREASE OF THE TOTAL SOLIDIFICATION TEMPERATURE
IN SALT MEDIA BY DEXTRAN PROTECTION

Todor Donev* and Snezhana Dimitrova

National Bank for Industrial Microorganisms and Cell Cultures,
 1113 Sofia, P.O. Box 239, Bulgaria
 
 

Summary

        An investigation was performed on the possibilities for an alteration of the total solidification temperature when preserving concentrated salt media (MBM, MEM, 199, HAM), used for the maintenance of animal viruses and cell cultures. It had been established that by the use of dextran 40000, this temperature could be increased with about 20oC. A protection in 3.6% concentration was recommended and the freezing to minus 40oC might be performed directly in the refrigerator for storage. The temperature should not be raised over minus 32oC during the stockage.
 

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