JOURNAL OF CULTURE COLLECTIONS
Volume 2, 1997-1998

 

CRYOPRESERVATION OF TOSPOVIRUSES

Angela Yordanova1*, Dimitrinka Christova2 and Todor Donev1

1National Bank for Industrial Microorganisms and Cell Cultures,
 Sofia 1113, P.O.Box 239, Bulgaria;
2Plant Protection Institute, 2230 Kostinbrod, Bulgaria
 
 

Summary

        Investigations for conservation in liquid nitrogen of tospoviruses under the guise of infected leaf material and plant sap were performed. The protecting action of four cryoprotecting media was investigated . For reading the results an indicator test and ELISA were used. It had been registered that the specific biological properties and the virulent ability were fully preserved in the tospoviruses conserved with 10% dimethylsulphoxid and kept during three years in liquid nitrogen.
 

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