JOURNAL OF CULTURE COLLECTIONS
Volume 2, 1997-1998

 

ISOLATION AND CHARACTERISATION OF TWO THERMOTOLERANT KLUYVEROMYCES YEAST STRAINS

Anna Kujumdzieva1*, Trajana Nedeva1, Maria Morfova1 and Valentin Savov2

1The Sofia University, Biological Faculty, Department of General and Industrial Microbiology,
8 "Dragan Tsankov" st., 1421 Sofia, Bulgaria;
2The Sofia University, Biological Faculty, Department of Engineering Biology,
8 "Dragan Tsankov" st., 1421 Sofia, Bulgaria
 
 

Summary

        Two new thermotolerant lactose consuming yeast strains, producers of thermostabile superoxide dismutase (SOD) (EC: 1.15.1.1.) were isolated and taxonomically identified as Kluyveromyces marxianus var. lactis T1 and Kluyveromyces marxianus var. bulgaricus T3. The influence of carbon source and growth temperature on the specific growth rate and SOD biosynthesis was also studied. Both strains grew well on lactose and/or lactose containing feedstocks and those substrates could be used for the production of SOD enzyme with high activity. Temperature of 40oC was proved to be suitable for cultivation of both strains with respect of SOD biosynthesis. Assay for thermostability of SOD enzyme of T1 and T3 strains cultivated on lactose shows that it is a thermostable one.
        The newly isolated and identified strains can be used as producers of thermostable SOD when grown on lactose and lactose containing feedstocks at elevated temperatures.

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