The trehalose accumulation by 77 superficially cultivated yeast strains from genus Saccharomyces was investigated in dynamics. The conditions were determined for the maximal achievement of the storage carbohydrate in the cells. Experiments with optimal duration of 120 - 144 hours were performed on two nutrient media. The brewery yeasts produced significantly more trehalose (10 - 13%) when compared with the wine and baking ones.