JOURNAL OF CULTURE COLLECTIONS
Volume 2, 1998-1999

 

NATURAL POLYMORPHISM OF STRAIN STREPTOMYCES GALBUS (F.)
SUBSP. ACHROMOGENES 695

Ljubomira Yocheva1* and Stefka Antonova-Nikolova2

1National Bank for Industrial Microorganisms and Cell Cultures,
1113 Sofia, P.O. Box 239, Bulgaria;
2The Sofia University, Biological Faculty, Department of Microbiology,
8 "Dragan Tsankov" st., 1421 Sofia, Bulgaria
 
 

Summary

        The natural variability of strain Streptomyces galbus (F) subsp. achromogenes 695 was examined. The changes of three characteristics: morphology of the colonies, formation of diffusible pigment and antibiotic activity, were studied on 21 agar media.
        It was established that the polymorphism of the strain was the greatest on Gauze's No1 medium. Its population divided into four morphological types of colonies and one subtype of type I, which differed in the development stage of the aerial mycelium and in the colour intensity of the aerial and vegetative mycelium and of the soluble pigment.
        A positive correlation between the antibiotic activity and the biosynthesis of diffusible pigment was observed.
        The variants of the basic (I) morphological type had the highest antibiotic activity. Strain 695-3, which activity was with 20% higher than the one of the initial culture, was chosen for further selection.
 
 

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