JOURNAL OF CULTURE COLLECTIONS
Volume 2, 1997-1998

 

LONG-TERM PRESERVATION OF VITALITY AND LIGHT EMISSION
OF LUMINOUS BACTERIAL STRAINS

Ignat Abrashev1, Anna Sotirova1, Konstantina Ilieva1 and Todor Donev2*

1Institute of Microbiology, Bulgarian Academy of Sciences,
"Acad. G. Bonchev" str., bl. 26, 1113 Sofia, Bulgaria;
2National Bank for Industrial Microorganisms and Cell Cultures,
 1113 Sofia, P.O.Box 239,Bulgaria
 
 

Summary

        It was established that lyophilization and cryogenic method were the most appropriate ones for preservation of luminous bacteria without changes in their morphology and properties. The strains kept their viability during a two years observation period. The reactivation of luminous bacteria, which were preserved under liquid paraffin, led to the restoration of their vitality and ability for light emission only in the course of 1 to 5 months.
 

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