LONG-TERM PRESERVATION OF VITALITY AND LIGHT EMISSION
OF LUMINOUS BACTERIAL STRAINS
Ignat Abrashev1, Anna Sotirova1, Konstantina Ilieva1 and Todor Donev2*
1Institute of Microbiology, Bulgarian Academy of Sciences,
"Acad. G. Bonchev" str., bl. 26, 1113 Sofia, Bulgaria;
2National Bank for Industrial Microorganisms and Cell Cultures,
1113 Sofia, P.O.Box 239,Bulgaria
Summary
It was established that
lyophilization and cryogenic method were the most appropriate ones for
preservation of luminous bacteria without changes in their morphology and
properties. The strains kept their viability during a two years observation
period. The reactivation of luminous bacteria, which were preserved under
liquid paraffin, led to the restoration of their vitality and ability for
light emission only in the course of 1 to 5 months.