INFLUENCE OF THE CULTIVATION CONDITIONS ON YEAST STRAINS SURVIVAL AFTER LYOPHILIZATION
Todor Donev1*, Irena Savova1 and Anna Kujumdzieva2
1National Bank for Industrial Microorganisms and Cell
Cultures,
1113 Sofia, P.O.Box 239, Bulgaria;
2The Sofia University, Biological Faculty, Department of
General and Industrial Microbiology,
8 "Dragan Tsankov" st., 1421 Sofia, Bulgaria
Summary
The growth curves of strains belonging to genera Candida and Saccharomyces grown through surfaced and submerged cultivation and their survival after lyophilization have been studied. Conclusions of practical importance have been drawn. It was found out that three-day-old cultures cultivated in solid medium possessed maximal resistance to freeze-drying. Nevertheless the cultures obtained by submerged cultivation were with considerably lower survival the best results were obtained at 12 h and 18 h of their development.