CHANGES IN STREPTOMYCES HYGROSCOPICUS 155 ENDOPEPTIDASE AND AMINOPEPTIDASE ACTIVITY AND HEAT RESISTANCE UNDER STARVATION AND INCREASED TEMPERATURE
Zdravka Sholeva1* and Iskra Ivanova2
1National Bank for Industrial Microorganisms and Cell Cultures,
1113 Sofia, P.O.Box 239, Bulgaria;
2 The Sofia University, Biological Faculty, Department of
General and Industrial Microbiology,
8 “Dragan Tsankov” st., 1421 Sofia, Bulgaria
Summary
The influence of temperature stress and starvation for amino acids, glucose and phosphates, on the heat resistance of mycelium and endo- and aminopeptidase activity of Streptomyces hygroscopicus 155, was studied. The strongest growth inhibition was determined at temperature elevation from 30o to 39oC and at starvation for amino acids. Also these stress treatments mostly induce the heat resistance of the mycelium. A correlation between the intracellular endo- and aminopeptidase activity and decrease in biomass yield was registered. The process of catabolization of proteins, during the adaptation to stress treatments, depends on energy and is stimulated by the presence of Mg2+ ions.